Engineered pH-buffering nanocomposite for robust one-pot multi-enzyme cascade synthesis of γ-aminobutyric acid

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Green Chem., 2025, Advance Article
DOI: 10.1039/D5GC02022K, Paper
Hang Luo, Tin Pou Lai, Yanxin Gao, Wenjin Li, Yilin Fan, Liyun Zhang
This work develops a PMAA@ZIF-8 nanocomposite that creates localized acidic microenvironments to overcome pH incompatibility in multi-enzyme cascades, enabling 93.6% GABA yield, efficient NADH regeneration and enhanced enzyme stability.
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