Integration of metabolic and bioprocess engineering for the production of β-ketoadipic acid from glucose and xylose by Pseudomonas putida

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Green Chem., 2025, Advance Article
DOI: 10.1039/D5GC01813G, Paper
Open Access Open Access
Gabriel M. Rubinstein, Sekgetho C. Mokwatlo, Louis Chirban, Ariel R. Lepard, Morgan A. Ingraham, Kelsey J. Ramirez, Hoon Choi, Patrick O. Saboe, Davinia Salvachúa, Christopher W. Johnson, Gregg T. Beckham
β-Ketoadipic acid, an intermediate in aerobic microbial aromatic catabolism suitable as a monomer in performance-advantaged biopolymers, was produced at a high titer, rate, and yield from glucose and xylose in P. putida with in situ product recovery.
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