Combining rational design and computational tools in multi-parameter enzyme engineering to increase the fitness of a CYP152 peroxygenase for α-hydroxylation of fatty acids

Green Chem., 2026, 28,7106-7116
DOI: 10.1039/D6GC01139J, Paper

Open Access

&nbsp This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Stephan Vrabl, Klara Bangert, Enrico F. Semeraro, Wolfgang Kroutil
The performance of the CYP152 peroxygenase PO_SPα was substantially improved for the α-hydroxylation of medium-chain fatty acids by mutating oxidation-sensitive solvent exposed amino acids and exploiting computational tools.
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