Novel iterative genome mining and engineering of a bifunctional KvVDH enable selective production of furan carboxylic acids from high-concentration 5-hydroxymethylfurfural

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Green Chem., 2026, Advance Article
DOI: 10.1039/D5GC06172E, Paper
Yanan Cui, Jie Zhang, Xijun Xing, Hanwen Zhang, Yutong Chen, Liqiang Fan, Xu Li, Yongjun Qiu, Chen Deng, Liming Zhao
In this study, an optimal mutant M3 (A50S/Y370W) with synergistic enhanced thermostability and activity was obtained, and a biocatalytic process was developed to efficiently and selectively oxidize 90–240 mM HMF into multiple furan carboxylic acids.
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