AUTHORS: Rajat Sharma1, Meinan Wang2, Xianming Chen2,3, Brett F. Carver4, Mary Guttieri5, Paul St. Amand5, Amy Bernardo5, Guihua Bai5, Anju Maan Ara1, and Meriem Aoun1* 1Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA 2Department of Plant Pathology, Washington State University, Pullman, WA, USA 3USDA-ARS Wheat Health, Genetics, and Quality Research Unit, Pullman, WA, USA 4Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, USA 5USDA-ARS Hard Winter Wheat Genetics Research Unit, Manhattan, KS, USA *CORRESPONDENCE: Meriem Aoun (meriem.aoun@okstate.edu) KEYWORDS: Stripe Rust Adult Plant Resistance (APR) Genotyping-by-Sequencing (GBS) Quantitative Trait Locus (QTL) Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is among the most destructive wheat diseases. Identifying resistance genes is crucial for the development of resistant cultivars. Bakers Ann, a hard winter wheat cultivar from Oklahoma State, has shown durable adult plant resistance to stripe rust. To dissect the genetic basis underlying stripe rust resistance in Bakers Ann, 125 doubled haploid lines, derived from the cross OK12D22004-016 X Bakers Ann, were evaluated at the adult plant stage in the greenhouse and in field environments in Oklahoma, Kansas, and Washington. This population was genotyped using genotyping-by-sequencing to produce single-nucleotide polymorphisms for genetic mapping. Quantitative trait locus (QTL) analysis identified six lociQTL, four from Bakers Ann on chromosomes 2DL, 4BS, 4BL, and 7BL, and two from OK12D22004-016 on chromosomes 2AS and 2AL. Although OK12D22004-016 is susceptible in the US Great Plains, it was found to carry QYr.osu-2AS on the 2NvS translocation that explained up to 30% of the phenotypic variation, but effective in a single location in Washington. Two major QTL were identified in Bakers Ann, QYr.osu-2DL on chromosome 2DL thatwhich explained up to 57% of the phenotypic variation and mapped close to Yr54, and QYr.osu-4BL on chromosome 4BL thatwhich explained up to 15% of the phenotypic variation and mapped close to Yr62. Resistance in Bakers Ann resulted from additive effects of the four QTL. Two allele-specific PCR markers were developed for QYr.osu-2DL to facilitate marker-assisted selection for stripe rust resistance.
Identification of stripe rust adult plant resistance genes in the hard winter wheat cultivar Bakers Ann
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