Harnessing deep eutectic solvents and mesoporous silica nanosphere immobilization for supercharged amine dehydrogenase catalysis in continuous flow

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Green Chem., 2026, Advance Article
DOI: 10.1039/D6GC01106C, Paper
Weixi Kong, Biao Li, Qi Zhang, Xuyang Lei, Ke Zheng, Xiaoyang Yue, Yunting Liu, Yanjun Jiang
Blocking a detrimental helix-to-loop transition by a betaine–acetic acid DES enhances engineered AmDH activity and stability, while immobilization and flow operations further augment these effects, enabling intensified ARA at high substrate loads.
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