Coupling a rebuild shuttle system with biosynthetic pathway and transcription factor engineering for enhanced L-cysteine production

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Green Chem., 2025, Advance Article
DOI: 10.1039/D5GC00433K, Paper
Bo Zhang, Xiaoling Xiu, Lifeng Chen, Xin Sui, Ruyi Zheng, Yuhe Guo, Xue Cai, Zhiqiang Liu, Yuguo Zheng
A novel plasmid-free engineered E. coli strain was developed to significantly increase L-cysteine yield, achieving a titer of 35.54 g L−1 in a 5 L bioreactor.
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