Structure-guided engineering of benzaldehyde lyase for efficient synthesis of dihydroxyacetone and one-pot biosynthesis of 2-amino-1,3-propanediol

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Green Chem., 2025, Advance Article
DOI: 10.1039/D5GC02006A, Paper
Yue Fan, Xuemeng Li, Yu Li, Jinhui Feng, Min Wang, Cuiying Zhang, Peiyuan Yao, Qiaqing Wu, Dunming Zhu
A novel formolase was discovered and engineered to efficiently catalyze the controllable transformation of formaldehyde into dihydroxyacetone, and a one-pot, two-step synthetic pathway was designed and implemented for the synthesis of serinol.
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